26 Influence of some amino acids on the dynamic swelling behavior of radiation-induced acrylamide hydrogel

Author(s): Saraydin, D; Oztop, HN; Karadag, E; Caldiran, Y; Guven, O

Title: Influence of some amino acids on the dynamic swelling behavior of radiation-induced acrylamide hydrogel

Source: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 82 (2): 115-125 NOV 1999

Language: English

Document Type: Article

Author Keywords: amino acid; hydrogel; acrylamide; swelling; diffusion

Keywords Plus: BOVINE SERUM-ALBUMIN; BIOCOMPATIBILITY; ADSORPTION; COPOLYMERS

Abstract: The influence of some amino acids-alanine, glycine, valine, glutamine, histidine, phenylalanine, and tryptophan-on the swelling behavior of acrylamide (AAm) hydrogel prepared by gamma-radiation was investigated. Swelling experiments of AAm hydrogel were made in the universal buffer solutions and the amino acid solutions at certain pHs at 37 degrees C. These selected pH values were pK(1), pK(2) and isoelectric point (pI) values such as ionization of alpha-carboxyl groups, ionization of alpha-amino groups, and the pls of the amino acids, respectively. The swelling of AAm hydrogel increased when pH values of solutions were increased. The value of equilibrium swelling of AAm hydrogel in the solution of universal buffer was 880% at pH 10.0, whereas it was 670% at pH 2.0. The values of equilibrium swelling of AAm hydrogel in amino acid solutions were between 830 and 965% at pH 10.0, whereas they were between 635 and 775% at pH 2.0. The rate constant of swelling, initial swelling rate, theoretical maximum swelling, diffusional exponent, network parameter, and diffusion coefficient were calculated by swelling kinetics. Diffusions of the amino acid solutions into the hydrogel were generally found as non-Fickian in character. The diffusion coefficients of the hydrogel were between 0.91 x 10(-6) and 2.41 x 10(-6) cm(2)/s.

Addresses: Cumhuriyet Univ, Dept Chem, TR-58140 Sivas, Turkey; Adnan Menderes Univ, Dept Chem, TR-09010 Aydin, Turkey; Hacettepe Univ, Dept Chem, TR-06532 Ankara, Turkey

Reprint Address: Saraydin, D, Cumhuriyet Univ, Dept Chem, TR-58140 Sivas, Turkey.

Cited References: BUCKLEY DJ, 1962, J POLYM SCI, V56, P163.
CRANK J, 1968, DIFFUSION POLYM.
GOPINATHAN C, 1989, B MATER SCI, V12, P49.
KARADAG E, 1994, POLYM ADVAN TECHNOL, V5, P664.
KARADAG E, 1996, BIOMATERIALS, V17, P67.
KARADAG E, 1997, J APPL POLYM SCI, V66, P733.
KATIME I, 1996, POLYM INT, V40, P281.
LI Y, 1990, J CHEM PHYS, V92, P1371.
LURIE I, 1975, HDB ANAL CHEM.
OZTOP HN, 1998, POLYM BULL, V40, P575.
PARKER L, 1988, LEARN MOTIV, V19, P1.
PENICHE C, 1997, POLYMER, V38, P5977.
PEPPAS NA, 1983, J POLYM SCI POL PHYS, V21, P983.
RITGER PL, 1987, J CONTROL RELEASE, V5, P23.
ROSKOSKI R, 1996, BIOCHEMISTRY.
SARAYDIN D, 1994, BIOMATERIALS, V15, P917.
SARAYDIN D, 1995, POLYM ADVAN TECHNOL, V6, P719.
SARAYDIN D, 1995, RADIAT PHYS CHEM, V46, P1049.
SARAYDIN D, 1997, POLYM J, V29, P631.
SARAYDIN D, 1998, POLYM BULL, V41, P577.
SHANKAR V, 1979, POLYMER, V20, P254.
SMITH EL, 1985, PRINCIPLES BIOCH.
WOERLY S, 1993, BIOMATERIALS, V14, P1056.

Cited Reference Count: 23

Times Cited: 7

Publisher: HUMANA PRESS INC

Publisher Address: 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA

ISSN: 0273-2289

29-char Source Abbrev.: APPL BIOCHEM BIOTECH

ISO Source Abbrev.: Appl. Biochem. Biotechnol.

Source Item Page Count: 11

Subject Category: BIOCHEMISTRY & MOLECULAR BIOLOGY; BIOTECHNOLOGY & APPLIED MICROBIOLOGY

ISI Document Delivery No.: 294PG