37 Immobilization of Saccharomyces cerevisiae on to radiation crosslinked HEMA/AAm hydrogels for production of ethyl alcohol

Author(s): Oztop, HN; Oztop, AY; Isikver, Y; Saraydin, D

Title: Immobilization of Saccharomyces cerevisiae on to radiation crosslinked HEMA/AAm hydrogels for production of ethyl alcohol

Source: PROCESS BIOCHEMISTRY, 37 (6): 651-657 JAN 10 2002

Language: English

Document Type: Article

Author Keywords: 2-hydroxyethyl methacrylate/acrylamide; hydrogel; radiation; immobilization; Saccharomyces cerevisiae; ethyl alcohol

Keywords Plus: ACRYLAMIDE/ITACONIC ACID HYDROGELS; BOVINE SERUM-ALBUMIN; YEAST-CELLS; CATIONIC DYES; SWELLING BEHAVIOR; AQUEOUS-SOLUTIONS; AMINO-ACIDS; ADSORPTION; BIOCOMPATIBILITY; POLYMERIZATION

Abstract: Radiation crosslinked 2-hydroxyethyl methacrylate/acrylamide (HEMA/AAm) copolymers prepared by gamma-irradiation were used in experiments on swelling, diffusion, immobilization of yeast cells (Saccharomyces cerevisiae) and production of ethyl alcohol. HEMA/AAm hydrogels irradiated at 3 kGy were used for swelling and diffusion studies in water and the nutrient medium of the cells. The parameters of equilibrium swelling, maximum swelling, initial swelling rate, diffusional exponent, network constant and diffusion coefficient of the hydrogel/penetrant systems were calculated and evaluated. All parameters for the hydrogel/water system were higher than that of the hydrogel/nutrient medium systems. Yeast cells (Saccharomyces cerevisiae) were immobilized on to the hydrogels by adsorption during multiplication and ethyl alcohol production of the hydrogels was investigated. Swelling of HEMA increased with the addition of AAm and ethyl alcohol production increased with increasing AAm in the hydrogels. (C) 2002 Elsevier Science Ltd. All rights reserved.

Addresses: Cumhuriyet Univ, Dept Chem, TR-58140 Sivas, Turkey; Cumhuriyet Univ, Fac Med, Microbiol & Clin Microbiol Dept, TR-58140 Sivas, Turkey

Reprint Address: Oztop, HN, Cumhuriyet Univ, Dept Chem, TR-58140 Sivas, Turkey.

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Cited Reference Count: 38

Times Cited: 3

Publisher: ELSEVIER SCI LTD

Publisher Address: THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND

ISSN: 0032-9592

29-char Source Abbrev.: PROCESS BIOCHEM

ISO Source Abbrev.: Process Biochem.

Source Item Page Count: 7

Subject Category: ENGINEERING, CHEMICAL; BIOCHEMISTRY & MOLECULAR BIOLOGY; BIOTECHNOLOGY & APPLIED MICROBIOLOGY

ISI Document Delivery No.: 523VG