40 pH-sensitive chitosan films for baker's yeast immobilization

Author(s): Oztop, HN; Saraydin, D; Cetinus, S

Title: PH-sensitive chitosan films for baker's yeast immobilization

Source: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 101 (3): 239-249 JUN 2002

Language: English

Document Type: Article

Author Keywords: yeast; cell; immobilization; chitosan

Keywords Plus: BEHAVIOR; DELIVERY; HYDROGEL

Abstract: Dried baker's yeast cells were immobilized on a chitosan film, which is a natural polymer. Prepared chitosan films were treated with glutaraldehyde to facilitate the immobilization of the cells. The effects of the amount of glutaraldehyde, incubation time, pH, and temperature on immobilization were investigated. The amount of glutaraldehyde was chosen to be 0.01% (weight). The highest amount of yeast immobilization was obtained with 5 h incubation. It was determined that optimum temperature for immobilization is 25degreesC, and the optimum pH for immobilization is 6. Immobilized cells were allowed to stand for 3 d in distilled water and buffer solution (pH 6) to investigate the desorption, but no desorption was found. The maximum immobilization capacities were found to be 90 mug protein cm(-2) film in optimum conditions.

Addresses: Cumhuriyet Univ, Dept Chem, Biochem Res Lab, TR-58140 Sivas, Turkey; Cumhuriyet Univ, Dept Chem, Hydrogel Res Lab, TR-58140 Sivas, Turkey

Reprint Address: Oztop, HN, Cumhuriyet Univ, Dept Chem, Biochem Res Lab, TR-58140 Sivas, Turkey.

Cited References: AYKUT G, 1988, BIOMATERIALS, V9, P168.
BARDI EP, 1996, J AGR FOOD CHEM, V44, P463.
CETINUS SA, 2000, ENZYME MICROB TECH, V26, P497.
GONG HP, 2000, J BIOMED MATER RES, V52, P285.
GUPTA KC, 2000, BIOMATERIALS, V21, P1115.
KARADAG E, 2001, J MACROMOL SCI PURE, V38, P1105.
KUMAR MNVR, 2000, REACT FUNCT POLYM, V46, P1.
LONG DD, 1996, J MACROMOL SCI PURE, V33, P1975.
MI FL, 2001, BIOMATERIALS, V22, P165.
OZTOP HN, 1998, POLYM BULL, V40, P575.
OZTOP HN, 2002, PROCESS BIOCHEM, V37, P651.
PAUL W, 2000, STP PHARMA SCI, V10, P5.
PRAKASHAM RS, 1999, APPL BIOCHEM BIOTECH, V82, P127.
SABNIS S, 2000, INT J BIOL MACROMOL, V27, P181.
SARAYDIN D, 1999, APPL BIOCHEM BIOTECH, V82, P115.
SCOPES KR, 1984, PROTEIN PURIFICATION, P1.
SEKI M, 1993, J CHEM ENG JPN, V26, P662.
TUAL C, 2000, J POLYM SCI POL PHYS, V38, P1521.
XIN LZ, 1992, RADIAT PHYS CHEM, V40, P579.
YAMAMOTO A, 2000, STP PHARMA SCI, V10, P23.
YAO KD, 1993, J APPL POLYM SCI, V48, P343.

Cited Reference Count: 21

Times Cited: 1

Publisher: HUMANA PRESS INC

Publisher Address: 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA

ISSN: 0273-2289

29-char Source Abbrev.: APPL BIOCHEM BIOTECH

ISO Source Abbrev.: Appl. Biochem. Biotechnol.

Source Item Page Count: 11

Subject Category: BIOCHEMISTRY & MOLECULAR BIOLOGY; BIOTECHNOLOGY & APPLIED MICROBIOLOGY

ISI Document Delivery No.: 566UK