46 Immobilization of Saccharomyces cerevisiae on to acrylamide-sodium acrylate hydrogels for production of ethyl alcohol

Author(s): Oztop, HN; Oztop, AY; Karadag, E; Isikver, Y; Saraydin, D

Title: Immobilization of Saccharomyces cerevisiae on to acrylamide-sodium acrylate hydrogels for production of ethyl alcohol

Source: ENZYME AND MICROBIAL TECHNOLOGY, 32 (1): 114-119 JAN 2 2003

Language: English

Document Type: Article

Author Keywords: acrylamide; hydrogel; immobilization; Saccharomyces cerevisiae; ethyl alcohol; sodium acrylate

Abstract: Acrylamide/sodium acrylate (AAm/SA) copolymers, prepared by using various crosslinkers, were used in experiments on swelling, diffusion, immobilization of yeast cells (Saccharomyces cerevisiae) and production of ethyl alcohol. AAm/SA hydrogels were used for swelling and diffusion studies in the nutrient medium of the cells. The parameters of equilibrium swelling, maximum swelling, initial swelling rate, diffusional exponent, network constant and diffusion coefficient of the hydrogel/penetrant systems were calculated and evaluated. Yeast cells were immobilized onto the hydrogels by adsorption method during multiplication and ethyl alcohol production of the hydrogels was investigated. Swelling of AAm increased with the addition of SA and ethyl alcohol production increased with increasing SA in the hydrogels. The best system for immobilization is found to be AAm/SA hydrogels containing N,N'-methylenebisacrylamide as crosslinker. (C) 2002 Elsevier Science Inc. All rights reserved.

Addresses: Cumhuriyet Univ, Dept Chem, Biochem Res Lab, TR-58140 Sivas, Turkey; Cumhuriyet Univ, Fac Med, Dept Microbiol Clin Microbiol, TR-58140 Sivas, Turkey; Adnan Menderes Univ, Dept Chem, TR-09010 Aydyn, Turkey; Cumhuriyet Univ, Dept Chem, Hydrogel Res Lab, TR-58140 Sivas, Turkey

Reprint Address: Oztop, HN, Cumhuriyet Univ, Dept Chem, Biochem Res Lab, TR-58140 Sivas, Turkey.

Cited References: AYKUT G, 1988, BIOMATERIALS, V9, P168.
BERNT E, 1974, METHOD ENZYMAT AN, P1499.
FUJIMURA T, 1987, BIOTECHNOL BIOENG, V29, P171.
FUJIMURA T, 1988, RADIAT PHYS CHEM, V31, P653.
ISONO Y, 1995, PROCESS BIOCHEM, V30, P743.
JEN AC, 1996, BIOTECHNOL BIOENG, V50, P357.
KARADAG E, 2002, TURK J CHEM, V26, P863.
KRAJEWSKA B, 1991, ACTA BIOTECHNOL, V11, P269.
NAVRATIL M, 1999, BIOLOGIA, V54, P635.
OZTOP HN, 2002, PROCESS BIOCHEM, V37, P651.
ROSIAK JM, 1999, NUCL INSTRUM METH B, V151, P56.
ROSIAK JM, 1999, RADIAT PHYS CHEM, V55, P139.
SARAYDIN D, 2002, PROCESS BIOCHEM, V37, P1351.
SRERE NK, 2000, J IND MICROBIOL BIOT, V24, P222.
VERVOORT L, 1998, INT J PHARM, V172, P127.

Cited Reference Count: 15

Times Cited: 4

Publisher: ELSEVIER SCIENCE INC

Publisher Address: 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA

ISSN: 0141-0229

29-char Source Abbrev.: ENZYME MICROB TECHNOL

ISO Source Abbrev.: Enzyme Microb. Technol.

Source Item Page Count: 6

Subject Category: BIOTECHNOLOGY & APPLIED MICROBIOLOGY

ISI Document Delivery No.: 632WJ