61 Immobilization of catalase onto chitosan and cibacron blue F3GA attached chitosan beads

Author(s): Cetinus SA (Cetinus, Senay Akkus), Oztop HN (Oztop, H. Nursevin), Saraydin D (Saraydin, Dursun)
Title: Immobilization of catalase onto chitosan and cibacron blue F3GA attached chitosan beads
Source:  ENZYME AND MICROBIAL TECHNOLOGY, 41 (4): 447-454 SEP 3 2007
Language: English
Document Type: Article
Author Keywords: chitosan beads; cibacron blue F3GA; catalase; adsorption; immobilization
Keywords Plus: TRIAZINE DYES; ADSORPTION; AFFINITY; FILM

Abstract: In this study, chitosan beads (Ch-bead) and cibacron blue F3GA attached chitosan beads (CB-Ch-bead) were prepared. Their characteristics were investigated with experiments of swelling, thermogravimetric analysis and Fourier transform infrared (FTIR) spectroscopic analysis. Catalase (CAT) was immobilized onto these beads. The adsorption isotherms have a Langmuirian shape for Ch-beads and CB-Ch-beads. The CAT adsorption capacity of Ch-beads is higher than that of CB-Ch-beads, but CB-Ch-CAT showed better activity according to the Ch-CAT. The values of apparent &, were found to be 18 and 41 mM for Ch-CAT and CB-Ch-CAT, respectively. However, V-max values were calculated as 4800 and 14,250 mu mol (mg protein)(-1) min(-1) for Ch-CAT and CB-Ch-CAT, respectively. Furthermore, various characteristics of immobilized catalase, such as the temperature profile, thermal stability, optimum pH, operational stability and storage stability were evaluated. Optimum temperature and optimum pH values were found as 35 degrees C and 7.0 for maximum activity of Ch-CAT and CB-Ch-CAT. It was observed that thermal, storage and operational stabilities of the enzyme were increased with immobilization. (c) 2007 Elsevier Inc. All rights reserved.

Addresses: 1. Cumhuriyet Univ, Fac Sci & Literature, Dept Chem Biochem, TR-58140 Sivas, Turkey
2. Cumhuriyet Univ, Fac Sci & Literature, Dept Chem Polymer Chem, TR-58140 Sivas, Turkey
Reprint Address:  Cetinus, SA (reprint author), Cumhuriyet Univ, Fac Sci & Literature, Dept Chem Biochem, TR-58140 Sivas, Turkey
E-mail Address: scetinus@cumhuriyet.edu.tr
Cited References:
ANCA MY J MEMBRANE SCI 129 : 65 1997

ARICA MY Immobilization of catalase in poly(isopropylacrylamide-co-hydroxyethylmethacrylate) thermally reversible hydrogels POLYMER INTERNATIONAL 48 : 879 1999

BERTILSSON L Cibacron Blue F3G-A anchored monolayers with biospecific affinity for NAD(H)-dependent lactate dehydrogenase: characterization by FTIR-spectroscopy and atomic force microscopy BIOSENSORS & BIOELECTRONICS 12 : 839 1997

BRADFORD MM RAPID AND SENSITIVE METHOD FOR QUANTITATION OF MICROGRAM QUANTITIES OF PROTEIN UTILIZING PRINCIPLE OF PROTEIN-DYE BINDING ANALYTICAL BIOCHEMISTRY 72 : 248 1976

CETINUS SA Immobilization of catalase into chemically crosslinked chitosan beads ENZYME AND MICROBIAL TECHNOLOGY 32 : 889 DOI 10.1016/S0141-0229(03)00065-6 2003

CETINUS SA Immobilization of catalase on chitosan film ENZYME AND MICROBIAL TECHNOLOGY 26 : 497 2000

CHATTERJEE U GOAT LIVER CATALASE IMMOBILIZED ON VARIOUS SOLID SUPPORTS JOURNAL OF FERMENTATION AND BIOENGINEERING 70 : 429 1990

CHEN CH Affinity chromatography of proteins on non-porous copolymerized particles of styrene, methyl methacrylate and glycidyl methacrylate JOURNAL OF CHROMATOGRAPHY A 921 : 31 2001

DEAN PDG PROTEIN-PURIFICATION USING IMMOBILIZED TRIAZINE DYES JOURNAL OF CHROMATOGRAPHY 165 : 301 1979

DENIZLI A Dye-ligand affinity systems JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS 49 : 391 2001

ERTAS N Specific determination of hydrogen peroxide with a catalase biosensor based on mercury thin film electrodes TURKISH JOURNAL OF CHEMISTRY 24 : 95 2000

GILES CH STUDIES IN ADSORPTION .11. A SYSTEM OF CLASSIFICATION OF SOLUTION ADSORPTION ISOTHERMS, AND ITS USE IN DIAGNOSIS OF ADSORPTION MECHANISMS AND IN MEASUREMENT OF SPECIFIC SURFACE AREAS OF SOLIDS JOURNAL OF THE CHEMICAL SOCIETY : 3973 1960

KENNEDY JF IMMOBILIZED ENZYMES AND CELLS CHEMICAL ENGINEERING PROGRESS 86 : 81 1990

LI SJ Use of chemically modified PMMA microspheres for enzyme immobilization BIOSYSTEMS 77 : 25 DOI 10.1016/j.biosystems.2004.03.001 2004

LOWE CR SOME PREPARATIVE AND ANALYTICAL APPLICATIONS OF TRIAZINE DYES INTERNATIONAL JOURNAL OF BIOCHEMISTRY 13 : 33 1981

MARTINS MCL Albumin and fibrinogen adsorption on Cibacron blue F3G-A immobilised onto PU-PHEMA (polyurethane-poly(hydroxyethylmethacrylate)) surfaces JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION 14 : 439 2003

OZTOP HN PH-sensitive chitosan films for baker's yeast immobilization APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 101 : 239 2002

SANTONI T Enzyme electrode for glucose determination in whole blood TALANTA 44 : 1573 1997

SCOPES KR PROTEIN PURIFICATION : 1 1984

SINGLA AK Chitosan: some pharmaceutical and biological aspects - an update JOURNAL OF PHARMACY AND PHARMACOLOGY 53 : 1047 2001

SOLAS MT IONIC ADSORPTION OF CATALASE ON BIOSKIN - KINETIC AND ULTRASTRUCTURAL STUDIES JOURNAL OF BIOTECHNOLOGY 33 : 63 1994

TUKEL SS Immobilization and kinetics of catalase onto magnesium silicate PROCESS BIOCHEMISTRY 39 : 2149 DOI 10.1016/j.procbio.2003.11.010 2004

TURNER AJ ISOLATION AND CHARACTERIZATION OF RAT-LIVER ALDEHYDE REDUCTASE BIOCHIMICA ET BIOPHYSICA ACTA 613 : 256 1980

ZHANG SP Further studies on the contribution of electrostatic and hydrophobic interactions to protein adsorption on dye-ligand adsorbents BIOTECHNOLOGY AND BIOENGINEERING 75 : 710 2001
Cited Reference Count: 24
Times Cited: 0
Publisher: ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
ISSN: 0141-0229
IDS Number: 199EV

DOI: 10.1016/j.enzmictec.2007.03.014