64 Acrylamide-Sepiolite Based Composite Hydrogels for Immobilization of Invertase

Author(s): Oztop HN (Oztop, H. Nursevin)1, Hepokur C (Hepokur, Ceylan)1, Saraydin D (Saraydin, Dursun)2
Source: JOURNAL OF FOOD SCIENCE    Volume: 74    Issue: 7    Pages: N45-N49    Published: SEP 2009  
Times Cited: 0     References: 22  
Abstract: Novel composite hydrogels, poly(acrylamide)-sepiolite (PAS), poly(acrylamide/acrylic acid)-sepiolite (PAAS), and poly(acrylamide/itaconic acid)-sepiolite (PAIS) were prepared and used for the immobilization of invertase. The parameters of equilibrium swelling, diffusional exponent, and diffusion coefficient of these hydrogels were calculated from swelling experiments. Invertase was immobilized onto PAS, PAAS, and PAIS and immobilized invertases (PASI, PAASI, and PAISI) were prepared. Optimum pH values for free invertase, PASI, PAASI, and PAISI are found to be 5, 5.5, 4.5, and 6, respectively, and the optimum temperatures were 30, 50, 50, and 35 degrees C for free invertase PASI, PAASI, and PAISI. It was found that K-m values of free invertase, PASI, PAASI, and PAISI were 11.3, 41.0, 94.5, and 56.0 mM, respectively. V-max values were 2 mm mol/min for free invertase, 8.10 mm mol/min for PASI, 1.30 mu mol/min for PAASI, and 0.42 mu mol/min for PAISI, respectively. The invertase immobilized hydrogels showed excellent, temperature, storage, and operational stability.
Document Type: Article
Language: English
Author Keywords: acrylamide; composite; immobilization; invertase; sepiolite
KeyWords Plus: GLUCOSE-OXIDASE; ADSORPTION
Reprint Address: Oztop, HN (reprint author), Cumhuriyet Univ, Dept Chem, Biochem Res Lab, TR-58140 Sivas, Turkey
Addresses:
1. Cumhuriyet Univ, Dept Chem, Biochem Res Lab, TR-58140 Sivas, Turkey
2. Cumhuriyet Univ, Dept Chem, Hydrogel Res Lab, TR-58140 Sivas, Turkey
E-mail Addresses: oztop@cumhuriyet.edu.tr
Funding Acknowledgement:
Funding Agency Cumhuriyet Univ. Research Fund
Publisher: WILEY-BLACKWELL PUBLISHING, INC, COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA
Subject Category: Food Science & Technology
IDS Number: 490JY
ISSN: 0022-1147
DOI: 10.1111/j.1750-3841.2009.01302.x